Promega nanoluc

Da, ATP- independent luciferase that utilizes a novel coelenterazine analog (furimazine) to produce high intensity, glow-type. The enzyme is about 100-fold brighter . Configured with NanoLuc Luciferase for use in reporter gene assays of transcriptional control—ideal for cloning of known or putative promoter region and . I would like to use a STATreporter plasmid for luciferase assays. Measures kinase target engagement in live cells.

Plant Chronobiologist previously at the Millar Lab. Currently working in the intersection of circadian dynamics, . NanoLuc is a novel, small and bright luciferase from the deep sea shrimp Oplophorus gracilirostris and developed by Promega. When compared to traditional . The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo . Nanoluc luciferase well suited as an.

A split NanoLuc assay system consisting of two fragments, large N-terminal and. Crystallographic structures of NanoLuc give a glimpse of. We have expressed the new reporter NanoLuc (Nluc) luciferase in.

Unless otherwise stated all bioluminescence reagents are from Promega. Monitoring intracellular protein interactions using NanoLuc. Corresponding author: brock.

NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells is the most downloaded article from ACS Chemical. A newly developed luciferase ( NanoLuc ) is 100–1× brighter than. Bret-based approach was developed by promega , and uses a fused nanoluciferase ( nanoluc ) at the n- terminal . Promega ) as template DNA for the NanoLuc moiety.